IgE tests using a
cellulose-solid phase can...
...contain varying amounts of CCDs sufficient
to cause false-positive test results. (1)
CCD-FREE PEANUT COMPONENTS
CCD False Positives Results
Despite using CCD-Free
CCD-Free Peanut Components
Figure 1: All samples were tested using CCD-free peanut components and nineteen of the ImmunoCAP positive samples exhibited a change in reactivity in the presence of a CCD inhibitor due to reactivity to the ImmunoCAP solid phase rather than any interaction with the proteins themselves, thereby creating false positive results (2).
Hemmer W, Altmann F, Holzweber F, et al. ImmunoCAP cellulose displays cross-reactive carbohydrate determinant (CCD) epitopes and can cause false-positive test results in patients with high anti-CCD IgE antibody levels. J Allergy Clin Immunol. 2018 Jan;141(1):372-381. DOI: 10.1016/j.jaci.2017.04.028.
Data on File. NOVEOS™ (HYCOR); ImmunoCAP (Thermo Fisher Scientific);