AUTOSTAT™ II ELISA Microplate Assay Kits

HYCOR offers more than 30 CE-marked clinically significant autoimmune serum markers for the diagnosis of human autoimmune diseases. HYCOR AUTOSTAT™ II ELISA assays can be performed manually or with the automated HYTEC™ 288 Plus enzyme immunoassay instrument.

  • Ready-to-use reagents in kits that include wash buffer concentrate
  • Microplates with 12 strips of 8 convenient break-apart wells
  • Horseradish peroxidase (HRP)/TMB detection system
  • Improved productivity and flexibility using the HYTEC 288 system: combine up to 8 different autoimmune parameters in one run

HYCOR AUTOSTAT II Declaration of Conformity with ISO/IEC Guide 22 Download here

Choose from:

Organ-specific autoantibodies

Part No. Antigen Specificity
FGA04 Anti-Thyroid Peroxidase (TPO)†: Thyroid
FGA03 Anti-Thyroglobolin (TG)†: Thyroid
FGA10 Anti-Gastric Parietal Cell: Stomach
FGA09 Anti-Mitochondrial (M2): Liver
FGA13 Anti-Glomerular Basement Membrane (GBM): Kidney

†Completely validated on DYNEX DSX instruments.
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Systemic autoantibodies

Part No. Antigen Specificity
FGA21 Anti-Nuclear Antibodies Screen (ANA)†
FGA14 ENA-6 Screen†: Extractable Nuclear Antigens

†Completely validated on DYNEX DSX instruments.
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ENA-6 (confirmation) tests

Part No. Antigen Specificity
FGA38 ENA-6-Profile* (16 cavities each for all of the 6 antigens)
FGA31 Anti-Sm†: Smith
FGA29 Anti-SS-A Ro†: Sjögren’s Syndrome
FGA30 Anti-SS-B La†: Sjögren’s Syndrome
FGA32 Anti-Sm / RNP†: Ribonuclear Protein
FGA34 Anti-Scl-70†: DNA Topoisomerase I, Systemic Sclerosis
FGA33 Anti-Jo-1†: Histidyl-tRNA-Synthetase

*Intended for manual application only.
†Completely validated on DYNEX DSX instruments.
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DNA autoantibodies

Part No. Antigen Specificity
FGA01 Anti-dsDNA†: Anti-double-stranded DNA
FGA02 Anti-ssDNA: Anti-single-stranded DNA

†Completely validated on DYNEX DSX instruments.
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ANCA (anti-nuclear cytoplasmic antibodies)

Part No. Antigen Specificity
FGA12 Anti-MPO p-ANCA: Myeloperoxidase, Peri-nuclear
FGA11 Anti-Pr-3 c-ANCA: Proteinase-3, Cytoplasmic

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Phospholipid autoantibodies

Part No. Antigen Specificity
FGA18 Anti-Cardiolipin Screen
FGA37 Anti-Cardiolipin Isotype* (IgA, IgG, IgM)
FGA15 Anti-Cardiolipin IgG
FGA16 Anti-Cardiolipin IgM
FGA24 Anti-ß2-Glycoprotein-I IgA
FGA22 Anti-ß2-Glycoprotein-I IgG
FGA23 Anti-ß2-Glycoprotein-I IgM

*Intended for manual application only.
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Rheumatoid factor

Part No. Antigen Specificity
FGA08 Total Rheumatoid Factor†
FGA06 Rheumatoid Factor IgA†
FGA07 Rheumatoid Factor IgG†
FGA05 Rheumatoid Factor IgM†

†Completely validated on DYNEX DSX instruments.
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Celiac disease autoantibodies

Part No. Antigen Specificity
FGA19 Anti-Gliadin IgG†
FGA20 Anti-Gliadin IgA†
FGA25 Tissue Transglutaminase (tTG) IgA†
FGA26 Tissue Transglutaminase (tTG) IgG†

†Completely validated on DYNEX DSX instruments.
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Circulating immune complexes

Part No. Antigen Specificity
FGA27 Circulating Immune Complex (CIC) C1q
FGA28 Circulating Immune Complex (CIC) C3d*

*Intended for manual application only.
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Basic reagents for autoimmune applications‡

Part No. Description Pkg. Contents/Size
KLA Substrate Solution (TMB) 15 mL
312500 HYTEC Tubes, 9 × 45 mm 500
D26L1000 Autoimmune Stop Solution (0.25 M H2SO4 ) 1000 mL
D17L1000 Autoimmune Wash Buffer (Standard) 1000 mL
D50L1000 Autoimmune Wash Buffer (Cardiolipin) 1000 mL

‡Only additionally required for the HYTEC™ 288 applications.
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